LAG3 antibody (N-Term)

Catalog No. ABIN1169105

Product Details anti-LAG3 Antibody

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Binding Specificity: N-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LAG3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP), Functional Studies (Func)

Specificity: Recognizes human LAG-3.

Cross-Reactivity: Human

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.001EU/μg purified protein or <1EU/mg purified protein (LAL test, Lonza).

Immunogen: Synthetic peptide corresponding to 30 aa in the N-terminus of human LAG-3.

Clone: 17B4

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-LAG3 antibody (ABIN1169105)

Triebel, Hacene, Pichon: "A soluble lymphocyte activation gene-3 (sLAG-3) protein as a prognostic factor in human breast cancer expressing estrogen or progesterone receptors." in: Cancer letters, Vol. 235, Issue 1, pp. 147-53, (2006) (PubMed).

Maçon-Lemaître, Triebel: "The negative regulatory function of the lymphocyte-activation gene-3 co-receptor (CD223) on human T cells." in: Immunology, Vol. 115, Issue 2, pp. 170-8, (2005) (PubMed).

Di Carlo, Cappello, Sorrentino, DAntuono, Pellicciotta, Giovarelli, Forni, Musiani, Triebel: "Immunological mechanisms elicited at the tumour site by lymphocyte activation gene-3 (LAG-3) versus IL-12: sharing a common Th1 anti-tumour immune pathway." in: The Journal of pathology, Vol. 205, Issue 1, pp. 82-91, (2004) (PubMed).

Demeure, Wolfers, Martin-Garcia, Gaulard, Triebel: "T Lymphocytes infiltrating various tumour types express the MHC class II ligand lymphocyte activation gene-3 (LAG-3): role of LAG-3/MHC class II interactions in cell-cell contacts." in: European journal of cancer (Oxford, England : 1990), Vol. 37, Issue 13, pp. 1709-18, (2001) (PubMed).

Hannier, Tournier, Bismuth, Triebel: "CD3/TCR complex-associated lymphocyte activation gene-3 molecules inhibit CD3/TCR signaling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 8, pp. 4058-65, (1998) (PubMed).

Huard, Mastrangeli, Prigent, Bruniquel, Donini, El-Tayar, Maigret, Dréano, Triebel: "Characterization of the major histocompatibility complex class II binding site on LAG-3 protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 11, pp. 5744-9, (1997) (PubMed).

Huard, Gaulard, Faure, Hercend, Triebel: "Cellular expression and tissue distribution of the human LAG-3-encoded protein, an MHC class II ligand." in: Immunogenetics, Vol. 39, Issue 3, pp. 213-7, (1994) (PubMed).

Baixeras, Huard, Miossec, Jitsukawa, Martin, Hercend, Auffray, Triebel, Piatier-Tonneau: "Characterization of the lymphocyte activation gene 3-encoded protein. A new ligand for human leukocyte antigen class II antigens." in: The Journal of experimental medicine, Vol. 176, Issue 2, pp. 327-37, (1992) (PubMed).

Target Details for LAG3

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Alternative Name: LAG-3 (LAG3 Products)

Synonyms: CD223 antibody, LAG-3 antibody, Ly66 antibody, lymphocyte activating 3 antibody, lymphocyte-activation gene 3 antibody, LAG3 antibody, Lag3 antibody

Background: Lymphocyte activation gene 3 (LAG-3, CD223) plays an important role in negatively regulating T cell proliferation, function and homeostasis. It is required for maximal natural and induced regulatory T cell (Treg) function. LAG-3 is closely related to the T cell co-receptor CD4 and binds to MHC class II molecules but with a significantly higher affinity than CD4.

UniProt: P18627

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints