STS antibody (N-Term)

Catalog No. ABIN1169071

Product Details anti-STS Antibody

Target: STS (Steroid Sulfatase (STS))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Functional Studies (Func)

Specificity: Recognizes human and mouse Asc.

Cross-Reactivity: Human, Mouse (Murine)

Immunogen: Synthetic peptide corresponding to aa at the N-terminal human Asc.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-STS antibody (ABIN1169071)

Cao, Tian, Li, Lv, Han, Zhuang, Cheng, Gong, Ying, Jin, Gao: "Suppression of NLRP3 Inflammasome by Erythropoietin via the EPOR/JAK2/STAT3 Pathway Contributes to Attenuation of Acute Lung Injury in Mice." in: Frontiers in pharmacology, Vol. 11, pp. 306, (2020) (PubMed).

Chen, Jiang, Liu, Li, Pei, Wang, Pan, Cui, Long, Wang, Zheng: "Melatonin alleviates intervertebral disc degeneration by disrupting the IL-1β/NF-κB-NLRP3 inflammasome positive feedback loop." in: Bone research, Vol. 8, pp. 10, (2020) (PubMed).

Liu, Lu, Chen, Muthuraj, Li, Pattabiraman, Zempleni, Kachman, Natarajan, Yu: "Protective Role of Shiitake Mushroom-Derived Exosome-Like Nanoparticles in D-Galactosamine and Lipopolysaccharide-Induced Acute Liver Injury in Mice." in: Nutrients, Vol. 12, Issue 2, (2020) (PubMed).

Rudloff, Ung, Dowling, Mansell, DAndrea, Ellisdon, Whisstock, Berger, Nold-Petry, Nold: "Parsing the IL-37-Mediated Suppression of Inflammasome Function." in: Cells, Vol. 9, Issue 1, (2020) (PubMed).

Deng, Guo, Tam, Johnson, Brickey, New, Lenox, Shi, Golenbock, Koller, McKinnon, Beutler, Ting: "Platelet-activating factor (PAF) mediates NLRP3-NEK7 inflammasome induction independently of PAFR." in: The Journal of experimental medicine, Vol. 216, Issue 12, pp. 2838-2853, (2020) (PubMed).

Sanchez-Lopez, Zhong, Stubelius, Sweeney, Booshehri, Antonucci, Liu-Bryan, Lodi, Terkeltaub, Lacal, Murphy, Hoffman, Tiziani, Guma, Karin: "Choline Uptake and Metabolism Modulate Macrophage IL-1β and IL-18 Production." in: Cell metabolism, Vol. 29, Issue 6, pp. 1350-1362.e7, (2020) (PubMed).

Guo, Liu, Jian, Cui, Fang, Zuo, Deng, Li, Wang, Zhao, Geng, Ouyang, Lai, Chen, Huang: "Nickel induces inflammatory activation via NF-κB, MAPKs, IRF3 and NLRP3 inflammasome signaling pathways in macrophages." in: Aging, Vol. 11, Issue 23, pp. 11659-11672, (2019) (PubMed).

Martínez-García, Martínez-Banaclocha, Angosto-Bazarra, de Torre-Minguela, Baroja-Mazo, Alarcón-Vila, Martínez-Alarcón, Amores-Iniesta, Martín-Sánchez, Ercole, Martínez, González-Lisorge et al.: "P2X7 receptor induces mitochondrial failure in monocytes and compromises NLRP3 inflammasome activation during sepsis. ..." in: Nature communications, Vol. 10, Issue 1, pp. 2711, (2019) (PubMed).

Panaitescu, Romero, Gomez-Lopez, Xu, Leng, Maymon, Pacora, Erez, Yeo, Hassan, Hsu: "In vivo evidence of inflammasome activation during spontaneous labor at term." in: The journal of maternal-fetal & neonatal medicine, Vol. 32, Issue 12, pp. 1978-1991, (2019) (PubMed).

Wang, Gong, Zhang, Li, Lu, Zhao, Yu, Wei, Yang, Liu, Yang, Li, Zhang: "NLRP3 Inflammasome Participates in Host Response to Neospora caninum Infection." in: Frontiers in immunology, Vol. 9, pp. 1791, (2019) (PubMed).

Zhong, Liang, Sanchez-Lopez, He, Shalapour, Lin, Wong, Ding, Seki, Schnabl, Hevener, Greenberg, Kisseleva, Karin: "New mitochondrial DNA synthesis enables NLRP3 inflammasome activation." in: Nature, Vol. 560, Issue 7717, pp. 198-203, (2019) (PubMed).

Gomez-Lopez, Romero, Xu, Garcia-Flores, Leng, Panaitescu, Miller, Abrahams, Hassan: "Inflammasome assembly in the chorioamniotic membranes during spontaneous labor at term." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 77, Issue 5, (2018) (PubMed).

Gomez-Lopez, Romero, Panaitescu, Leng, Xu, Tarca, Faro, Pacora, Hassan, Hsu: "Inflammasome activation during spontaneous preterm labor with intra-amniotic infection or sterile intra-amniotic inflammation." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 80, Issue 5, pp. e13049, (2018) (PubMed).

Lugrin, Martinon: "Detection of ASC Oligomerization by Western Blotting." in: Bio-protocol, Vol. 7, Issue 10, (2017) (PubMed).

Dang, McDonald, Russell, Cyster: "Oxysterol Restraint of Cholesterol Synthesis Prevents AIM2 Inflammasome Activation." in: Cell, Vol. 171, Issue 5, pp. 1057-1071.e11, (2017) (PubMed).

Fernández-Gil, Moneim, Ortiz, Shen, Soto-Mercado, Mendivil-Perez, Guerra-Librero, Acuña-Castroviejo, Molina-Navarro, García-Verdugo, Sayed, Florido, Luna, López, Escames: "Melatonin protects rats from radiotherapy-induced small intestine toxicity." in: PLoS ONE, Vol. 12, Issue 4, pp. e0174474, (2017) (PubMed).

Man, Karki, Sasai, Place, Kesavardhana, Temirov, Frase, Zhu, Malireddi, Kuriakose, Peters, Neale, Brown, Yamamoto, Kanneganti: "IRGB10 Liberates Bacterial Ligands for Sensing by the AIM2 and Caspase-11-NLRP3 Inflammasomes." in: Cell, Vol. 167, Issue 2, pp. 382-396.e17, (2017) (PubMed).

Tervaniemi, Katayama, Skoog, Siitonen, Vuola, Nuutila, Sormunen, Johnsson, Linnarsson, Suomela, Kankuri, Kere, Elomaa: "NOD-like receptor signaling and inflammasome-related pathways are highlighted in psoriatic epidermis." in: Scientific reports, Vol. 6, pp. 22745, (2016) (PubMed).

Man, Karki, Malireddi, Neale, Vogel, Yamamoto, Lamkanfi, Kanneganti: "The transcription factor IRF1 and guanylate-binding proteins target activation of the AIM2 inflammasome by Francisella infection." in: Nature immunology, Vol. 16, Issue 5, pp. 467-75, (2015) (PubMed).

Ortiz, Acuña-Castroviejo, Doerrier, Dayoub, López, Venegas, García, López, Volt, Luna-Sánchez, Escames: "Melatonin blunts the mitochondrial/NLRP3 connection and protects against radiation-induced oral mucositis." in: Journal of pineal research, (2014) (PubMed).

Target Details for STS

Target: STS (Steroid Sulfatase (STS))

Alternative Name: Asc (STS Products)

Background: Asc promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. It is a component of the inflammasome, a protein complex which also includes NLRP3/NALP3, CARD8 and CASP1 and whose function is the activation of proinflammatory caspases.

UniProt: Q9ULZ3

Pathways: Steroid Hormone Biosynthesis, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation