Recombinant Golgin B1 (GOLGB1) antibody

Catalog No. ABIN1169059

Product Details

Target: Golgin B1 (GOLGB1)

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Human

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunocytochemistry (ICC)

Specificity: Recognizes human and mouse giantin.

Cross-Reactivity: Human, Mouse (Murine)

Purification: Purified from HEK 293 cell culture supernatant.

Purity: >95 % (SDS-PAGE)

Immunogen: Human recombinant giantin.

Clone: TA10

Isotype: IgG2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

anti-Giantin, monoclonal antibody (recombinant) (TA10) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain. anti-Giantin, monoclonal antibody (recombinant) (TA10) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References

Vielemeyer, Nizak, Jimenez, Echard, Goud, Camonis, Rain, Perez: "Characterization of single chain antibody targets through yeast two hybrid." in: BMC biotechnology, Vol. 10, pp. 59, (2010) (PubMed).

Nizak, Martin-Lluesma, Moutel, Roux, Kreis, Goud, Perez: "Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo." in: Traffic (Copenhagen, Denmark), Vol. 4, Issue 11, pp. 739-53, (2003) (PubMed).

Target Details

Target: Golgin B1 (GOLGB1)

Alternative Name: Giantin (GOLGB1 Products)

Synonyms: gcp antibody, golim1 antibody, giantin antibody, 4930428L02Rik antibody, 628101 antibody, 6330407A06Rik antibody, AU042952 antibody, C130074L01Rik antibody, F730017E11Rik antibody, Gm6840 antibody, mKIAA4151 antibody, GCP antibody, GCP372 antibody, GOLIM1 antibody, golgin B1 antibody, golgin B1 S homeolog antibody, golgi autoantigen, golgin subfamily b, macrogolgin 1 antibody, GOLGB1 antibody, golgb1.S antibody, Golgb1 antibody

Background: Giantin, the largest Golgi membrane protein, is C-terminally anchored to cis- and medial-Golgi membranes and forms a long ~250nm coiled-coil rod that directly interacts with microtubules in a cell-cycle regulated way. It is an excellent marker for mammalian golgi complex. It is also an antigen in chronic rheumatoid arthritis and in the autoimmune disease Sjoegren syndrome.

UniProt: Q14789

Pathways: SARS-CoV-2 Protein Interactome