Laminin antibody (Biotin)

Catalog No. ABIN116904

Product Details anti-Laminin Antibody (Biotin)

Target: Laminin (LN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Laminin antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Characteristics: Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule.

Purification: Immunoaffinity chromatography.

Immunogen: Produced against a mixture of synthetic peptides corresponding to regions of adherence on laminin.

Application Details

Application Notes: Anti-Laminin antibodies have been used for indirect trapping ELISA for quantitation ofantigen in serum using a standard curve, for Immunoprecipitation and for Western blottingfor highly sensitive qualitative analysis. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against Laminin at a dilution of 1: 5,000 to 1: 10,000. This product was also assayedagainst 1.0 μg of Laminin in a standard capture ELISA using Peroxidase ConjugatedStreptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substratefor 30 minutes at room temperature. A working dilution of 1: 4,000 to 1: 8,000 of the stockconcentration is suggested for this product. For Immunohistochemistry on paraffinembedded tissue dilute the product 1: 50 to 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative and 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for Laminin

Target: Laminin (LN)

Alternative Name: Laminin (LN Products)

Synonyms: LAMC1 antibody, laminin antibody, laminin, beta 2 (laminin S) antibody, LAMB2 antibody