This antibody may react non-specifically with other proteins. This antibody is suitable for the detection by immunoblot of Human, Mouse and Rat IκB-alpha. Control Peptide (R1013CP) will compete only with the specific reaction of antiserum with IκB-alpha.
Purification
Prepared from monospecific antiserum by Delipidation and Defibrination.
Immunogen
IkBa peptide corresponding to a region near the C-terminus of the Human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
NFKBIA
Reactivity: Human, Mouse, Rat
WB, ELISA, IF (cc), IF (p), IHC (p), FACS, IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Suitable for Immunoblotting (1/2,000-1/10,000), ELISA (1/20,000-1/100,000) andImmunohistochemistry (1/1,000-1/5,000). Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against IκBa showing a 36 kDa band. Perform all incubations except colordevelopment using TBS supplemented with 0.1 % Tween-20 at room temperature. Block themembrane with 5 % dry milk for 2 h. Add a 1: 1,000 dilution of the primary antibody to themembrane and incubated for 2 h. Perform washes with buffer 4 times for 5' each. Incubatethe immunoblot with Peroxidase conjugated Affinity Purified Goat anti-Rabbit IgG [H&L] at1/2,000 for 1 h. Wash with TBS only preceded color development. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
80 mg/mL (by Refractometry)
Buffer
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.09 % (w/v) Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
NFKBIA
(Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))
IKBA antibody, MAD-3 antibody, NFKBI antibody, AI462015 antibody, Nfkbi antibody, RL/IF-1 antibody, ECI-6/IKBA antibody, NFKBIA antibody, ikbaB antibody, wu:fb81f08 antibody, zgc:56666 antibody, zgc:77616 antibody, ikbaA antibody, wu:fj16a07 antibody, zgc:85769 antibody, NFKB inhibitor alpha antibody, nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha antibody, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha b antibody, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha a antibody, NFKBIA antibody, Nfkbia antibody, nfkbiab antibody, nfkbiaa antibody
Background
Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IKB alpha and IKB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IKB is confirmed by immunoblotting.Synonyms: I kappa B-alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF-kappa-B inhibitor alpha, NFKBI