IL-3 beta antibody
IL-3 beta
Reactivity: Rat
WB, EIA, Func
Host: Goat
Polyclonal
unconjugated
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- Target See all IL-3 beta products
- IL-3 beta (Interleukin 3 beta (IL-3 beta))
- Reactivity
- Rat
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This IL-3 beta antibody is un-conjugated
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)
- Specificity
- Rat Interleukin-3 beta
- Cross-Reactivity (Details)
- Species reactivity (tested):Rat.
- Purification
- Affinity chromatography
- Immunogen
- Highly pure (>98%) recombinant rIL-3 beta.
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- Application Notes
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of RatIL-3beta (100 ng/mL), a concentration of 2.7-4.0 μg/mL of this antibody is required. ELISA: To detect rat IL-3 beta by direct ELISA (using 100 μL/well antibody solution) thisantibody can be used at a concentration of 0.15-0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinantrat IL-3 beta. Western Blot: To detect rat IL-3 beta by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant rat IL-3 beta is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL
- Buffer
- PBS, pH 7.2 without preservatives.
- Preservative
- Without preservative
- Handling Advice
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody at 2-8 °C for one month or at -20 °C for longer.
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- Target
- IL-3 beta (Interleukin 3 beta (IL-3 beta))
- Alternative Name
- Interleukin-3 beta / IL3 beta (IL-3 beta Products)
- Background
- IL3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells.Synonyms: IL-3 beta, IL3B
- Gene ID
- 24495
- NCBI Accession
- NP_113701
- UniProt
- P97688
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