Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman SCGF-beta (30.0 ng/mL), a concentration of 2.5-5.0 μg/mL of this antibody isrequired. Sandwich ELISA: To detect Human SCGF-beta by Sandwich ELISA (using 100 μL/wellantibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. Thisantigen affinity purified antibody, in conjunction with Biotinylated anti-Human SCGF-beta(PP1106B1 or PP1106B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human SCGF-beta. Western Blot: To detect Human SCGF-beta by Western Blot analysis this antibody can beused at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human SCGF-beta is 1.5-3.0 ng/lane, undereither reducing or non-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in sterile water to a concentration of 0.1-1.0 mg/mL
Buffer
PBS, pH 7.2 without preservatives.
Preservative
Without preservative
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody at 2-8 °C for up to one month or (in aliquots) at -20 °C for longer.
Target
CLEC11A
(C-Type Lectin Domain Family 11, Member A (CLEC11A))
This cytokine stimulates the proliferation and differentiation of hematopoietic precursor cells from various lineages, including erythrocytes, lymphocytes, granulocytes and macrophages. It acts synergistically with other cytokines, including IL3, GCSF, GMCSF and FLT3 ligand and suppresses SCF-stimulated erythrocyte proliferation.Synonyms: C-type lectin domain family 11 member A, C-type lectin superfamily member 3, CLECSF3, LSLCL, Lymphocyte secreted C-type lectin, Stem cell growth factor, p47