CXCL12 antibody (Biotin)
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- Target See all CXCL12 Antibodies
- CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CXCL12 antibody is conjugated to Biotin
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Specificity
- Recognizes Stromal Cell Derived Factor-1 alpha (SDF-1 alpha).
- Purification
- Affinity Chromatography.
- Immunogen
- Highly pure (>98%) E.coli derived recombinant Human SDF1-alpha.
- Top Product
- Discover our top product CXCL12 Primary Antibody
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- Application Notes
- ELISA: To detect hSDF-1alpha by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. This antibody, in conjunctionwith compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hSDF-1alpha. To detect hSDF-1alpha by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. This antibody, in conjunctionwith a polyclonal Anti-Human SDF-1alpha (PP1067P) as a capture antibody, allows thedetection of at least 0.2 - 0.4 ng/well of recombinant hSDF-1alpha. Western Blot: To detect hSDF-1alpha by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hSDF-1alpha is 1.5 - 3.0 ng/lane, under either reducingor non-reducing conditions.
- Restrictions
- For Research Use only
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- Reconstitution
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
- Buffer
- PBS, pH 7.2 without preservatives.
- Preservative
- Without preservative
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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TLR7/8 triggering exerts opposing effects in acute versus latent HIV infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 5, pp. 2888-95, (2006) (PubMed).
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TLR7/8 triggering exerts opposing effects in acute versus latent HIV infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 5, pp. 2888-95, (2006) (PubMed).
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- Target
- CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))
- Alternative Name
- CXCL12 / SDF1 (CXCL12 Products)
- Synonyms
- IRH antibody, PBSF antibody, SCYB12 antibody, SDF1 antibody, TLSF antibody, TPAR1 antibody, CINC-1 antibody, Gro1 antibody, Pbsf antibody, Scyb12 antibody, Sdf1 antibody, Tlsf antibody, Tpar1 antibody, Fsp antibody, KC antibody, Mgsa antibody, N51 antibody, Scyb1 antibody, gro antibody, sdf-1 antibody, sdf1 antibody, xSDF-1 antibody, SDF-1 antibody, cxcl12 antibody, sdf-1a antibody, sdf1a antibody, wu:fa55e10 antibody, wu:fc16h12 antibody, wu:fj84c02 antibody, C-X-C motif chemokine ligand 12 antibody, C-X-C motif chemokine ligand 1 antibody, chemokine (C-X-C motif) ligand 12 antibody, chemokine (C-X-C motif) ligand 1 antibody, C-X-C motif chemokine ligand 12 L homeolog antibody, chemokine (C-X-C motif) ligand 12a (stromal cell-derived factor 1) antibody, CXCL12 antibody, cxcl12 antibody, Cxcl1 antibody, Cxcl12 antibody, cxcl12.L antibody, cxcl12a antibody
- Background
- SDF1 alpha and 1 beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF or IL1. SDF1 alpha has been shown to be chemotactic for lymphocytes and is recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV1 entry.Synonyms: C-X-C motif chemokine 12, IRH, Intercrine reduced in hepatomas, PBSF, Pre-B cell growth-stimulating factor, SDF1A, SDF1B, Stromal cell-derived factor 1, hIRH
- Gene ID
- 6387
- NCBI Accession
- NP_000600
- UniProt
- P48061
- Pathways
- Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling
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