IL-1 beta antibody
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- Target See all IL-1 beta (IL1B) Antibodies
- IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
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Reactivity
- Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This IL-1 beta antibody is un-conjugated
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)
- Purification
- Affinity chromatography.
- Immunogen
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-1-beta.
- Top Product
- Discover our top product IL1B Primary Antibody
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- Application Notes
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofmIL-1-beta (50 pg/mL), a concentration of 100 - 150 ng/mL of this antibody is required. Elisa: To detect mIL-1-beta by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5g/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of0.2-0.4 ng/well of recombinant mIL-1-beta. Western Blot: To detect mIL-1-beta by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mIL-1-beta is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Add. Information: Centrifuge vial prior to opening. - Restrictions
- For Research Use only
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- Reconstitution
- Restore with sterile water to a concentration of 0.1-1.0 mg/mL.
- Concentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.4 without preservatives.
- Preservative
- Without preservative
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- The lyophilized antibody can be stored for one year from despatch at -20 °C. Reconstituted antibody is stable for two weeks at 2-8 °C, frozen aliquots are stable for six months at -20 °C.
- Expiry Date
- 12 months
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- Target
- IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
- Alternative Name
- Interleukin-1 beta / IL-1B (IL1B Products)
- Synonyms
- IL-1 antibody, IL1-BETA antibody, IL1F2 antibody, IL-1BETA antibody, IL1beta antibody, il1-b antibody, zgc:111873 antibody, IL-1B antibody, IL-1beta antibody, Il-1b antibody, IL1B antibody, IL-1 beta antibody, IL-1b antibody, interleukin 1 beta antibody, interleukin 1, beta antibody, IL1B antibody, il1b antibody, Il1b antibody
- Background
- Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence.Synonyms: Catabolin, IL-1 beta, IL1 beta, IL1B, IL1F2
- Gene ID
- 16176
- NCBI Accession
- NP_032387
- UniProt
- P10749
- Pathways
- NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome
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