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His Tag antibody

WB Host: Rabbit Polyclonal unconjugated

Catalog No. ABIN115691

Datasheet as PDF

Target See all His Tag Antibodies

His Tag

Reactivity

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Host
93

60

11

9

5

4

1

1
Rabbit
Compatible Secondaries
Clonality
95

76

10
Polyclonal
Conjugate
79

21

14

13

5

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This His Tag antibody is un-conjugated
Application
142

92

39

27

25

19

15

14

14

13

13

7

7

5

5

4

4

4

3

3

2

2

1

1

1
Western Blotting (WB)

Sequence

HHHHHH

Purification

Antigen Affinity Chromatography using a His-peptide as matrix.

Immunogen

Different highly purified 6x His-tagged proteins (C-terminal) produced in insect cells.

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anti-His Tag antibody
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anti-His Tag antibody
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anti-His Tag antibody
WB, ELISA, IP, IHC, FACS Host: Mouse Monoclonal 33D10-D2-G8 unconjugated

anti-His Tag antibody
WB, ELISA, IP, IF, FACS Host: Mouse Monoclonal 6G2A9 unconjugated

anti-His Tag antibody (HRP)
WB, ELISA, IP Host: Mouse Monoclonal 33D10-D2 HRP

anti-His Tag antibody
Reactivity: Human WB Host: Rabbit Polyclonal RB19960 unconjugated

anti-His Tag (C-Term) antibody
WB, ELISA Host: Rabbit Polyclonal unconjugated

anti-His Tag antibody (Biotin)
WB, ELISA, IP Host: Mouse Monoclonal 33D10-D2 Biotin

anti-His Tag antibody
WB, ELISA, IP, DB, ISt Host: Mouse Monoclonal HIS-H8 unconjugated

Application Notes

Western blot (0.5-1 μg/mL): The antibody was tested using several His-tagged proteinsexpressed in insect cells and E. coli.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Reconstitution

Restore in sterile water to a concentration of > 0.5 mg/mL.

Buffer

PBS, pH 7.4, without preservatives.

Preservative

Without preservative

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C/-20 °C

Storage Comment

The lyophilized IgG is stable at 2-8 °C for one month from despatch and for one year when kept at -20 °C. The reconstituted antibody can be stored at 2-8 °C for one month or at -20 °C for one year.

Expiry Date

12 months
Target

His Tag

Alternative Name

6xHistidine Epitope Tag (His Tag Products)

Target Type

Tag

Background

In the last couple of years many peptide sequences/epitopes for the purification of recombinant proteins have been established. These so-called