POSTN
Reactivity: Human
IHC
Host: Mouse
Monoclonal
MSVA-649M
unconjugated
Application Notes
ELISA. Western blot (0.1-1 μg/mL). Immunohistochemistry on Paraffin (1/100-1/5,000) and Frozen Sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Add 0.05 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Buffer
0.05 M Phosphate Buffer, 0.1 M NaCl, pH 7.2 without preservatives
Preservative
Without preservative
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Stable the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Periostin is a disulfide linked 90 kDa 811 amno acid protein originally isolated as a osteoblast-specific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP)-2. OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.Synonyms: OSF-2, OSF2, Osteoblast-specific factor 2, PN, POSTN