Chromogranin A antibody (C-Term)

Catalog No. ABIN115294

Product Details anti-Chromogranin A Antibody

Target: Chromogranin A (CHGA)

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Chromogranin A antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody reacts with human Chromogranin A in the neuroendocrine cells and neural tissues. It does not recognizes the epitopes located on the N-terminal half of the chromogranin A molecule.
Cellular Localization: Cytoplasmic.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Purified

Immunogen: C-terminal half of chromogranin A was purified from urine of patients with carcinoid syndrome.

Application Details

Application Notes: Immunohistochemistry on Paraffin Embedded Sections: 1/20-1/50 in an ABC method(30-60 minutes at room temperature)Recommended Positive Control: Pancreas.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM Citrate buffer, pH 6.0 containing Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Chromogranin A

Target: Chromogranin A (CHGA)

Alternative Name: Chromogranin A (CHGA Products)

Background: Chromogranin A (a protein of 439 amino acids which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, phenochromocytomas, paragangliomas, and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.Synonyms: CHGA, CgA, Chromogranin-A, Pituitary secretory protein I, SP-I

Gene ID: 1113

NCBI Accession: NP_001266

UniProt: P10645

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling