This antibody reacts with Von Willebrand factor in endothelial cells. It also reacts with megakaryocytes in human bone marrow. Cellular Localization: Cytoplasmic.
Immunohistochemistry on Paraffin Embedded Sections: Use Factor VIII R ag antibody at1/15-1/30 dilution in an ABC method (30 minutes at RT). Proteolytic enzyme digestion of formalin fixed paraffin embedded tissue sections isrequired prior to immunostaining. Recommended Positive Control: Tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.