CEACAM1 antibody

Catalog No. ABIN114594

Product Details anti-CEACAM1 Antibody

Target: CEACAM1 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1))

Reactivity: Human, Mammalian

Host: Mouse

Clonality: Monoclonal

Conjugate: This CEACAM1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects complete native protein expressed on transfected cells.

Purification: Protein G Chromatography

Immunogen: Genetic immunisation with cDNA encoding the extracellular region of Human CEACAM1-A2. Based on recognition of the complete native protein expressed on transfected mammalian cells.

Clone: 8G5

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1.2 μg/10^6 cellsCompetitive ELISA: 1: 200 - 1: 400. ELISA: 1: 200 - 1: 400. Immunoflourescence. Immunohistology: 10 μg/mL (on cryosections).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the reconstituted antibody at 2-8 °C for one month (add 0.09% Sodium Azide) or at -20 °C for longer.

References for anti-CEACAM1 antibody (ABIN114594)

Peng, Oberst, Huang, Brohawn, Morehouse, Lekstrom, Baeuerle, Wu, Yao, Coats, DallAcqua, Damschroder, Hammond: "The CEA/CD3-bispecific antibody MEDI-565 (MT111) binds a nonlinear epitope in the full-length but not a short splice variant of CEA." in: PLoS ONE, Vol. 7, Issue 5, pp. e36412, (2012) (PubMed).

Sémiramoth, Gleizes, Turbica, Sandré, Gorges, Kansau, Servin, Chollet-Martin: "Escherichia coli type 1 pili trigger late IL-8 production by neutrophil-like differentiated PLB-985 cells through a Src family kinase- and MAPK-dependent mechanism." in: Journal of leukocyte biology, Vol. 85, Issue 2, pp. 310-21, (2009) (PubMed).

Béaslas, Torreilles, Casellas, Simon, Fabre, Lacasa, Delers, Chambaz, Rousset, Carrière: "Transcriptome response of enterocytes to dietary lipids: impact on cell architecture, signaling, and metabolism genes." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 295, Issue 5, pp. G942-52, (2008) (PubMed).

Nittka, Böhm, Zentgraf, Neumaier: "The CEACAM1-mediated apoptosis pathway is activated by CEA and triggers dual cleavage of CEACAM1." in: Oncogene, Vol. 27, Issue 26, pp. 3721-8, (2008) (PubMed).

Thomas, Zhu, Schnaar, Alves, Konstantopoulos: "Carcinoembryonic antigen and CD44 variant isoforms cooperate to mediate colon carcinoma cell adhesion to E- and L-selectin in shear flow." in: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 15647-55, (2008) (PubMed).

Yoon, Terada, Kita: "CD66b regulates adhesion and activation of human eosinophils." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 12, pp. 8454-62, (2007) (PubMed).

Avadhanula, Rodriguez, Devincenzo, Wang, Webby, Ulett, Adderson: "Respiratory viruses augment the adhesion of bacterial pathogens to respiratory epithelium in a viral species- and cell type-dependent manner." in: Journal of virology, Vol. 80, Issue 4, pp. 1629-36, (2006) (PubMed).

Kammerer, Riesenberg, Weiler, Lohrmann, Schleypen, Zimmermann: "The tumour suppressor gene CEACAM1 is completely but reversibly downregulated in renal cell carcinoma." in: The Journal of pathology, Vol. 204, Issue 3, pp. 258-67, (2004) (PubMed).

Target Details for CEACAM1

Target: CEACAM1 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1))

Alternative Name: CD66a / CEACAM1 (CEACAM1 Products)

Synonyms: BGP antibody, BGP1 antibody, BGPI antibody, CD66e antibody, CEA antibody, zeh0068 antibody, hm:zeh0068 antibody, CEACAM8 antibody, CEACAM1 antibody, MGC142545 antibody, BGPR antibody, Bgp antibody, CD66a antibody, Ccam1 antibody, Bgp1 antibody, C-CAM antibody, Cc1 antibody, Cea-1 antibody, Cea-7 antibody, Cea1 antibody, Cea7 antibody, Hv-2 antibody, Hv2 antibody, MHVR antibody, MHVR1 antibody, Mhv-1 antibody, bb-1 antibody, mCEA1 antibody, mmCGM1 antibody, mmCGM1a antibody, mmCGM2 antibody, carcinoembryonic antigen related cell adhesion molecule 1 antibody, carcinoembryonic antigen related cell adhesion molecule 5 antibody, carcinoembryonic antigen-related cell adhesion molecule 1 antibody, carcinoembryonic antigen-related cell adhesion molecule 6 antibody, CEACAM1 antibody, CEACAM5 antibody, ceacam1 antibody, Ceacam1 antibody, LOC101103343 antibody

Background: CEACAM1 (BGP/CD66a) is a transmembrane glycoprotein which belongs to the carcinoembryonic antigen (CEA) gene family (1,2). It is expressed on cells of epithelial and myeloid origin and mediates homophilic intercellular interactions that influence cellular growth, immune cell activation, and tissue morphogenesis. CEACAM1 is a putative tumour suppressor based on diminished expression in some aggressive types of cancer cells (3). The anti-tumour effect may be due to inhibition of tumour angiogenesis, possibly by increased secretion of anti-angiogenic molecules from the cells (4). Like all members of the CEACAM family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by two immunoglobulin constant-like A (A1, A2) and one B domain. While the N, A1 and B domains can also be found in other CEA-family members, the A2 domain of CEACAM1 differs from those found in other CEACAM.Synonyms: BGP, BGP1, Biliary glycoprotein 1, Carcinoembryonic antigen-related cell adhesion molecule 1

Gene ID: 634

NCBI Accession: NP_001020083

UniProt: P13688