Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

MHC Class I (RT1Ac) antibody (Biotin)

Reactivity: Rat FACS, IHC (fro) Host: Mouse Monoclonal OX-27 Biotin
Catalog No. ABIN114391
  • Target See all MHC Class I (RT1Ac) products
    MHC Class I (RT1Ac)
    Reactivity
    Rat
    Host
    • 7
    Mouse
    Clonality
    • 7
    Monoclonal
    Conjugate
    • 3
    • 2
    • 1
    • 1
    This MHC Class I (RT1Ac) antibody is conjugated to Biotin
    Application
    • 7
    • 2
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Purification
    Protein A Chromatography
    Immunogen
    Phytohaemagglutinin Blasts Donor: BALB/c spleen Fusion Partner: P3-NS1-1-Ag4 (NS1/1)
    Clone
    OX-27
    Isotype
    IgG2a
  • Application Notes
    Flow Cytometry. Immunohistochemistry using cryostat sections: (however, cross-reactivity with Lewis ratshave been shown to occur in some instances).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protocol
    FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-Rat cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0. 5-1. 0 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (Streptavidin-FITC) at a 1: 500 dilution. Notrecommended for use with a PE secondary. 9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protectedfrom light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Rat Strain: Brown NorwayCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 1. 0 µg/10e6 cellsIsotypic Control: Biotin Mouse IgG2aCell Source Percentage of cells stained above control: Thymus 65. 4%Spleen 98. 9%Lymph Node 100%Restults - Strain Distribution: Cell Concentration: 1x10e6 cells per testAntibody Concentration Used: 1. 0 µg/10e6 cellsStrains Tested: Brown Norway, Buffalo, Wistar, Fischer, ACIPositive: Brown NorwayNegative: Buffalo, Wistar, Fischer, ACI
    Restrictions
    For Research Use only
  • Concentration
    0.1 mg/mL
    Buffer
    PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    MHC Class I (RT1Ac)
    Abstract
    MHC Class I (RT1Ac) Products
    Background
    MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.Synonyms: MHC class I RT1.Ac heavy chain, RT1-A3
    Gene ID
    10116
    UniProt
    Q31255
You are here:
Support