Keratin 33A antibody

Catalog No. ABIN113469

Product Details anti-Keratin 33A Antibody

Target: Keratin 33A (KRT33A)

Reactivity: Human, Mouse

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This Keratin 33A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: The antiserum stains specifically Human hair keratin K33 expressed starting from the middle hair cortex. Because of high sequence homology both Keratin isoforms K33a (formerly also designated keratin hHa3-I) and K33b (formerly designated keratin hHa3-II) are detected.

Purification: Liquid serum

Immunogen: Synthetic peptide of human hair (trichocytic) keratin hHa3 (EKP IGS CVT NPC GPR SR), coupled to KLH.

Application Details

Application Notes: Immunohistochemistry on frozen sections: 1/200, 1h at RT, preincubate fixed sections with0.1 Triton X-100 (in PBS) for 1 - 5 min prior to first antibody incubation step. Immunoblotting: 1 : 2,000, ECL method.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for Keratin 33A

Target: Keratin 33A (KRT33A)

Alternative Name: Keratin-33A (KRT33A) (KRT33A Products)

Background: Synonyms: HHA3-I, HKA3A, Hair keratin type I Ha3-I, K33A, KRTHA3A, Keratin type I cuticular Ha3-I

Gene ID: 3883

NCBI Accession: NP_004129

UniProt: O76009