The antibody reacts specifically with The LDL Receptor (160 kDa mature or glycosylated receptor, 120 kDa precursor or unglycosylated receptor) plays a key role in cellular cholesterol homeostasis. It does not inhibit binding of LDL.
Cross-Reactivity (Details)
Species reactivity (tested):Human.
Purification
Immunoaffinity Chromatography
Immunogen
Specific synthetic peptide (sequence not conserved in VLDL receptor and LRP) of the LDL receptor extracellular domain (epitope between residues 184-195, the linker region between repeats 4 and 5)
LDLR
Reactivity: Rat
WB, IHC, ICC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Flow Cytometry: 1/10. Immunobloting: 1/100-1/200. Recognizes the 160 kDa band of LDLR and a 120 kDa band ofthe LDLR precursor from Fibroblasts, Hepatocytes, and monocytic cells cultured in thepresence of lipoprotein-deficient serum. Immunohistochemistry: 1/20. Receptor Binding Studies. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.1 mg/mL
Buffer
PBS, 0.5 % BSA
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Store the lyophilized antibody at -20 °C. Following reconstitution, the product ist stable at 2-8 °C for up one week or (in aliquots) at -20 °C for up to six months.
Akazawa, Date, Morikawa, Murayama, Miyamoto, Kaga, Barth, Baumert, Dubuisson, Wakita: "CD81 expression is important for the permissiveness of Huh7 cell clones for heterogeneous hepatitis C virus infection." in: Journal of virology, Vol. 81, Issue 10, pp. 5036-45, (2007) (PubMed).
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Background
The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemiaSynonyms: LDL receptor, LDLR, Low-density lipoprotein receptor