Complement C4A/C4B antibody

Catalog No. ABIN113341

Product Details

Target: Complement C4A/C4B

Reactivity: Human

Host: Chicken

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Enzyme Immunoassay (EIA)

Specificity: This antibody was found not to cross-react with Human C3a. The antibody was generated against the carboxy-terminal neoepitope which is not exposed on C4, allowing discrimination between C4 and C4a.

Purification: Affinity Chromatography

Immunogen: Synthetic peptide coupled to carreir protein.

Application Details

Application Notes: ELISA: The titer with native human C4a was > 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, 0.09 % Sodium Azide, 30 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Target Details

Target: Complement C4A/C4B

Synonyms: C4 antibody, C4-1 antibody, C4b antibody, complement component 4A (Rodgers blood group) antibody, C4a antibody

Background: The complement system is part of the innate immune system representing humoral defence. Activation of the complement cascade via the the alternative pathway liberates several biologically active by-products, among them the anaphylatoxin C4a. Measurement of C4a (and C3a) in plasma or other body fluids can yield important clues about the pathological, and particularly also the inflammatory status of an organism. In serum, C4a activity is under control of the carboxypeptidase N. This enzyme rapidly cuts off the C-terminal arginine, thereby generating C4adesArg. This degradation product C4adesArg remains relatively stable until it is cleared from the circulation.Synonyms: Acidic complement C4, Basic complement C4, CPAMD2, CPAMD3, Complement component 4

Gene ID: 720

NCBI Accession: NP_009224

UniProt: P0C0L4