HBSAg antibody (FITC)

Catalog No. ABIN112969

Product Details anti-HBSAg Antibody (FITC)

Target: HBSAg (HBsAg) (Hepatitis B Virus Surface Antigen (HBsAg))

Reactivity: Hepatitis B Virus (HBV)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HBSAg antibody is conjugated to FITC

Application: Immunofluorescence (IF)

Purification: Affinity Chromatography on Protein A .

Immunogen: Hepatitis B surface antigen purified from human serum. Mixture of subtypes ad & ay.

Application Details

Application Notes: Direct IFA staining of target antigens in a permissive tissue culture system. Acetone fixation of the antigen source is recommended prior to staining.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 4-5 mg/mL (OD280 nm, E0.1%= 1.4)

Buffer: 0.01 M PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative and 10 mg/mL BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Do not freeze working dilutions

Target Details for HBSAg

Target: HBSAg (HBsAg) (Hepatitis B Virus Surface Antigen (HBsAg))

Alternative Name: Hepatitis B Surface Antigen / HBsAg (HBsAg Products)

Target Type: Viral Protein

Background: Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.Synonyms: HBV surface antigen, Hepatitis B Virus