CTXB antibody (HRP)
CTXB
Reactivity: Vibrio cholerae
EIA, WB, IHC (fro)
Host: Rabbit
Polyclonal
HRP
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- Target See all CTXB products
- CTXB (CtxB Gene Product (CTXB))
- Reactivity
- Vibrio cholerae
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CTXB antibody is conjugated to HRP
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Application
- Enzyme Immunoassay (EIA), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- This antibody detects the beta subunit of Cholera toxin.
- Immunogen
- Purified Choleragenoid.
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- Application Notes
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Immunohistochemistry on frozen sections (1: 20 - 1: 200). ELISA (1: 200 - 1: 1000). Western blot (1: 20 - 1: 200).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1-2 mg/mL
- Buffer
- Phosphate buffered saline, pH 7.2, containing 0.002 % Thimerosal and 1 % Bovine serum albumin
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- CTXB (CtxB Gene Product (CTXB))
- Alternative Name
- Cholera Enterotoxin Subunit B (CTXB Products)
- Background
- The Beta subunit of cholera toxin binds to a GM1-ganglioside receptor which is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin Alpha subunit into cells. The holotoxin consists of a pentameric ring of Beta subunits whose central pore is occupied by the Alpha subunit. The Alpha subunit contains two chains, A1 and A2, linked by a disulfide bridge. The Alpha subunit (and Cholera toxin) activate the adenylate cyclase enzyme in cells of the intestinal mucosa leading to increased levels of intracellular cAMP.Synonyms: Cholera enterotoxin B chain, Cholera enterotoxin gamma chain, Choleragenoid, VC_1456, ctxB, toxB
- Gene ID
- 2613962
- NCBI Accession
- NP_231099
- UniProt
- P01556
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