Suitable for use in Double-Diffusion and CIE, direct IFA, ELISA and Immunohistochemistry. Use neat in gel-precipitin reactions. Direct FA staining of target antigens in a permissive tissue culture system. Acetone fixation of the antigen source is recommended prior to staining. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
4-5 mg/mL (OD280 nm, E0.1% = 1.4)
Buffer
0.01 M PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative and 10 mg/mL BSA as stabilizer
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Vanhee, Meersseman, Lagrou, Maertens, Nelis, Coenye: "Rapid and direct quantification of viable Candida species in whole blood by use of immunomagnetic separation and solid-phase cytometry." in: Journal of clinical microbiology, Vol. 48, Issue 4, pp. 1126-31, (2010) (PubMed).
Candida albicans is the most frequently isolated fungal pathogen of humans, affecting immunocompromised patients ranging from premature infants to AIDS sufferers. Systemic infections have an attributed mortality of 30-50 % . C. albicans is a diploid organism which has eight sets of homologous chromosomes. It has a genome of approximately 16 Mb (haploid), about 30 % greater than S. cerevisiae (baker's yeast).