Transferrin antibody (N-Term)

Catalog No. ABIN112496

Product Details anti-Transferrin Antibody

Target: Transferrin (TF)

Binding Specificity: N-Term

Reactivity: Human, Rat, Pig, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transferrin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificity: This antibody is specific for the N-terminal domain of Transferrin and is known to block the binding of this antibody to Transferrin. HTF-14 recognises a band of approximately 77 kDa under reducing conditions in Human plasma. Clone HTF-14 is not able to recognize receptor bound Transferrin. Clone HTF-14 recognizes a conformational epitope in a loop of the N lobe of Transferrin involving the critical Lys-144 residue, also essential for binding of the N lobe to the transferrin receptor (Mason et al.2009) Transferrin is a 77 kDa plasma protein synthesized by the liver involved with the transport of iron. Each transferrin molecule has two domains, both with facility to carry 2 ferric ions. The iron/transferrin complex is essential for haemoglobin synthesis and certain types of cell division. Negative Species. Bovine, Sheep, Canine (Dog) and Equine (Horse).

Cross-Reactivity (Details): Species reactivity (expected):Porcine, Rat and Rabbit.
Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein A

Immunogen: Purified Porcine Transferrin.

Clone: HTF-14

Isotype: IgG1

Application Details

Application Notes: ELISA. Immunofluorescence. Functional Application.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Transferrin

Target: Transferrin (TF)

Abstract: TF Products

Background: Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 mmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH . Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.Synonyms: Beta-1 metal-binding globulin, Serotransferrin, Siderophilin

Gene ID: 7018

NCBI Accession: NP_001054

UniProt: P02787

Pathways: Transition Metal Ion Homeostasis