This antibody is specific for an epitope within the extracellular domain, epitope locus I. It recognizes both the reduced and native non-reduced human and primate tissue factors. It does not inhibit coagulation or neutralize factor VII binding to CD142.
Purification
Affinity Chromatography on Protein G.
Immunogen
Denatured Tissue factor isolated from human brain by the Factor VII affinity method.
ELISA. Western blot. Flow Cytometry. Immunofluorescence. Immunohistology on Frozen and Paraffin Sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
Phosphate buffered saline containing 0.09 % Sodium Azide as preservative and 200 mM Mannitol as stabilizer.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
Tissue factor (F3)
(Coagulation Factor III (thromboplastin, Tissue Factor) (F3))
CD142, also known as Tissue factor, is the membrane receptor for coagulation factors VII and VIIa and is the cell surface initiator of coagulation. It is the major molecule of this type and is criticial for controlling hemostasis, thrombosis and inflammation.Synonyms: Coagulation factor III, F3, TF, Thromboplastin