MFGE8
Reactivity: Human
ELISA, IHC
Host: Mouse
Monoclonal
2B2D10
unconjugated
Application Notes
Immunohistochemistry on methanol/acetone fixed cultured cells and on cryostat orformalin fixed paraffin-embedded sections (strongly positive on human epithelia-basedstructures). ELISA. Western Blot. Immunocytochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH ~7.4, 15 mM Sodium Azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage
4 °C/-20 °C
Storage Comment
Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
MFGE8
(Milk Fat Globule-EGF Factor 8 Protein (MFGE8))
Human Milk Fat Globule 1 (MFG1) is a membrane protein present on normal human breast epithelial cells and cell lines derived from breast carcinomas. Human MFG1 is also localised on the surface of the human milk fat globule. HMFG1 is a differentiation marker and specific breast epithelial marker in normal and neoplastic mammary development. MFG1 Binds specifically to rotavirus and inhibits its replication.Synonyms: Breast epithelial antigen BA46, HMFG, MFGE8, MFGM, Milk fat globule-EGF factor 8