M13 Bacteriophage G8P Capsid antibody
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- Target
- M13 Bacteriophage G8P Capsid
- Reactivity
- Bacteriophage M13
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
- Specificity
- RL-ph1 reacts with the M13 filamentous phage coat protein g8p with a Molecular Weight of approx. 5 kDa.
- Purification
- Purified
- Immunogen
- Isolated M13 phage coat proteins. Remarks: Derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse.
- Clone
- RL-ph1
- Isotype
- IgG2b
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- Application Notes
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ABIN112397 is particularly useful for Immunoblotting of separated phage proteins(1/100-1/1000), Immunocytochemistry, Flow cytometry (1/25-1/200),Immunohistochemistry (1/25-1/200 with ABC as detection system), ELISA, Affinitychromatography.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS, 0.09 % Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the lyophilized antibody at 2-8 °C for one month or at -20 °C for longer. After reconstitution aliquot and store at -20 °C.
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- Target
- M13 Bacteriophage G8P Capsid
- Target Type
- Phage Protein
- Background
- The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.Synonyms: Coat protein B, Gene 8 protein, Major coat protein
- Gene ID
- 927333
- NCBI Accession
- NP_510890
- UniProt
- P69541
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