Keratin 3 antibody

Catalog No. ABIN112318

Product Details anti-Keratin 3 Antibody

Target: Keratin 3 (KRT3)

Reactivity: Human, Rabbit, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Cytokeratin 3 antibody, clone AE5 represents an excellent marker for corneal type differentiation. Positive for epithelial cells of cornea, snout and some oral mucosa. This antibody has been used for studying corneal epithelial stem cells. Polypeptide Reacting: Mr 64 000 polypeptide (Keratin K3, formerly also designated Cytokeratin 3) of human corneal epithelium and Keratin K76 (formerly also designated Cytokeratin K2p) of palate epithelium. Reactivities on Cultured Cell Lines: Rabbit corneal epithelial cells. In Cow and Rabbit it reacts with lip and snout epithelia.

Cross-Reactivity (Details): Species reactivity (tested):Human, Rabbit and Bovine.

Purification: Ion Exchange Chromatography.

Immunogen: Human epithelial keratin

Clone: AE5

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: 1/500. Immunohistochemistry on Frozen Sections: 1/50 (1h at RT).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7,2 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Keratin 3

Target: Keratin 3 (KRT3)

Alternative Name: Cytokeratin 3 (KRT3 Products)

Synonyms: CK3 antibody, K3 antibody, CK-3 antibody, keratin 3 antibody, KRT3 antibody

Background: Cytokeratin 3 belongs to the intermediate filament family. It is a type II cytokeratin and is specifically expressed in the corneal epithelium. Cytokeratin 3 associates with Cytokeratin 12. Defects in Cytokeratin 3 are a cause of Meesmann corneal dystrophy.Synonyms: 65 kDa cytokeratin, KRT3, Keratin, Type-II keratin Kb3, keratin-3, keratin-76, type II cytoskeletal 3

Gene ID: 3850

NCBI Accession: NP_476429

UniProt: P12035