Uroplakin 3A antibody

Catalog No. ABIN112250

Product Details anti-Uroplakin 3A Antibody

Target: Uroplakin 3A (UPK3A)

Reactivity: Human, Rat, Cow, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Uroplakin 3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Clone AU1 reacts specifically with Uroplakin III present in the superficial cell layer of the urothelium. The binding region has been localized on the extracellular part of the antigen. Antibody AU1 strongly stains the urothelial surface membrane in paraffin sections of human renal pelvis, ureter, bladder, and urethra. About 60 % of human transitional cell carcinomas (including metastases) maintain focal (sometimes very limited) expression of Uroplakin III. Until now, no Uroplakin staining was found in any non-urothelial carcinoma (Moll et al., 1995). Uroplakin III may thus serve as a specific urothelial differentiation marker in cases of metastatic carcinomas with unclear primary tumor.

Cross-Reactivity (Details): Species reactivity (tested):Human, Bovine, Pig and Rat.

Purification: Affinity Chromatography on Protein A.

Immunogen: Asymmetric unit membrane (AUM) preparation from bovine urinary bladder.

Clone: AU1

Isotype: IgG1

Application Details

Application Notes: Immunoblotting (Western blotting). Immunohistochemistry on Paraffin-Embedded Tissue (not on Frozen Sections! ) using aheat-induced retrieval method (e. g. microwave treatment): Working dilution: 1/10. Incubation Time: 1h at RT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL distilled water.

Buffer: Final solution contains PBS, pH 7.4 with 0.5 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for Uroplakin 3A

Target: Uroplakin 3A (UPK3A)

Alternative Name: Uroplakin-3a (UPK3A) (UPK3A Products)

Background: Together with the Uroplakins UP Ia, UP Ib and UP II, Uroplakin III contributes in constituting the asymmetrical unit membrane of the plaques of urothelial superficial (umbrella) cells. Uroplakin is a membrane glycoprotein (47 kD) and has been shown to be a specific marker of terminal urothelial differentiation (Wu et al., 1993, 1994).Synonyms: UP3a, UPIII, UPK3, Uroplakin III

Gene ID: 7380

NCBI Accession: NP_001161046

UniProt: O75631