Reacts with 111 kDa (form "a") and 103 kDa (form "b") polypeptides (Desmocollin 1 splice forms, both at pI 5.2) from Human epidermal desmosomes. Mab localizes Dsc1 present in - suprabasal layers of interfollicular epidermis - specific cell layers (e.g. in hair follicle root sheath) - Hassall bodies (spheroidal formations of densely packed reticulum-derived cells in the thymus).
Purification
Affinity Chromatography on Protein A
Immunogen
Synthetic peptide corresponding to sequence present within the intracellular part of Human Desmocollin 1
DSC1
Reactivity: Human, Mouse, Rat
ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Imunoblotting. Immunohistochemistry on frozen sections: 1: 10, 1 h at RT or overnight at 2-8C,preincubation with 0.05 - 0.2 % Triton X-100, for 5-10 min, depending on tissue type, isrecommended, Without detergent pretreatment a cytoplasmic component mightcross-react (e. g. in fibroblasts). Immunohistochemistry on paraffin-embedded sections (after microwave treatment): 1: 10, 1h at RT or overnight at 2-8C, a slight crossreactivity with a lymphocyte subpopulation isabolished by mild trypsinization after the microwave step) Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in 1 mL dist. water.
Buffer
PBS buffer, pH 7.4 containing 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Desmocollin 1 is a component of intercellular desmosome junctions. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. It may contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms. Desmocollin 1 is linked to the keratinization of epithelial tissues.Synonyms: CDHF1, Cadherin family member 1, DG2/DG3, DSC1, Desmosomal glycoprotein 2/3