ADRP antibody (N-Term)

Catalog No. ABIN112185

Product Details anti-ADRP Antibody

Target: ADRP (PLIN2) (Perilipin 2 (PLIN2))

Binding Specificity: AA 5-27, N-Term

Reactivity: Human, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This ADRP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Polypeptide Reacting: Adipophilin / ADRP, MW 48,100 (calculated from aa sequence data), apparent Mr 52,000 (after SDS-PAGE), pI 6.72. Tissue Immunolocalization: Adipophilin is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver, adipocytes are negative. Also positively stained are lipid-storing CD 68-positive macrophages. Tested Reactivity on Cultured Cell lines: Caco, PLC, HaCat, SV80, RD 125, Huvec (human umbilical cord endothelia), RV, PC-12 (rat adrenal gland), MDCK. Negative with glioma.

Cross-Reactivity (Details): Species reactivity (tested):Human, Rat and Dog. Negative with Bovine and Mouse.

Purification: Affinity Chromatography

Immunogen: Synthetic peptide corresponding to aa 5-27 from N-terminus of Human Adipophilin.

Clone: AP125

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on Frozen Tissue: 1/10 in PBS, pH 7.4(See Ref.17 for staining protocols). Immunohistochemistry on Paraffin-Embedded Tissue (After microwave treatment). Incubate 1 h at room temperature or over night at 2-8 °C. Cytological Material. Immunoblotting (Western blot): 0.2 μg/mL using ECL has been reported for previous batchnumbers.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL distilled water

Buffer: Final solution contains 0.09 % Sodium Azide and 0.5 % BSA in PBS buffer, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C.

References for anti-ADRP antibody (ABIN112185)

Leitner, Hlavatý, Ertl, Gabner, Fuchs-Baumgartinger, Walter: "Lipid droplets and perilipins in canine osteosarcoma. Investigations on tumor tissue, 2D and 3D cell culture models." in: Veterinary research communications, Vol. 46, Issue 4, pp. 1175-1193, (2022) (PubMed).

Leitner, Hlavaty, Heider, Ertl, Gabriel, Walter: "Lipid droplet dynamics in healthy and pyometra-affected canine endometrium." in: BMC veterinary research, Vol. 18, Issue 1, pp. 221, (2022) (PubMed).

Breher-Esch, Sahini, Trincone, Wallstab, Borlak: "Genomics of lipid-laden human hepatocyte cultures enables drug target screening for the treatment of non-alcoholic fatty liver disease." in: BMC medical genomics, Vol. 11, Issue 1, pp. 111, (2019) (PubMed).

Skwarek-Maruszewska, Hotulainen, Mattila, Lappalainen: "Contractility-dependent actin dynamics in cardiomyocyte sarcomeres." in: Journal of cell science, Vol. 122, Issue Pt 12, pp. 2119-26, (2009) (PubMed).

Ohsaki, Maeda, Fujimoto: "Fixation and permeabilization protocol is critical for the immunolabeling of lipid droplet proteins." in: Histochemistry and cell biology, Vol. 124, Issue 5, pp. 445-52, (2005) (PubMed).

Target Details for ADRP

Target: ADRP (PLIN2) (Perilipin 2 (PLIN2))

Alternative Name: Adipophilin / ADFP (PLIN2 Products)

Background: Adipophilin / ADRP is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines (see e.g. Heid et al., for review see e.g. Targett-Adams et al.), inducible by etomoxir. Enhanced expression of Adipophilin / ADRP is a useful marker for pathologies characterized by increased lipid droplet accumulation. Such diseases include atheroma, steatosis, obesity and certain cases of liposarcoma. It also seems to be a potent marker for atherosclerosis. ADRP can also be used to study the virus entry of e.g. HCV via lipid droplets (see e.g. Hope et al.).Synonyms: ADRP, Adipose differentiation-related protein

Gene ID: 123

NCBI Accession: NP_001113

UniProt: Q99541

Pathways: Regulation of Lipid Metabolism by PPARalpha, Lipid Metabolism