Cytokeratin 5 antibody (FITC)

Catalog No. ABIN112169

Product Details anti-Cytokeratin 5 Antibody (FITC)

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Reactivity: Human, Mouse, Cow, Pig, Hydra, Amphibian

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 5 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: C22 represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. The antibody specifically reacts with keratins K5 and K8 present in nearly all epithelia. Polypeptide reacting: Mr 52 500, Mr 58 000 keratins (type II keratins K5 and K8, formerly also designated cytokeratins 5 and 8) of human epithelial cells. Epitope has been mapped to aa 353-367 on alpha helical rod domain (see Waseem et al., 2004). Reactivities on Cultured Cell Lines (tested so far): MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12, bovine BMGE+H, BMGE-H, MDBK.

Cross-Reactivity (Details): Also reacts to: KRT8

Purification: Affinity Chromatography on Protein A.

Immunogen: Human Keratin K8, purified from SDS PAGE gel

Clone: C22

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry. Immunocytology. Immunohistochemistry on Frozen Tissues (1/10). Immunohistochemistry on Paraffin-Embedded Tissues (1/10). Incubation Time: 1 h at RT, extended with paraffin sections (overnight at 2-8 °C). Pretreatment: With paraffin-embedded sections, protease pretreatment is required prior to

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Cytokeratin 5

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Alternative Name: Cytokeratin 5 (KRT5 Products)

Background: Cytokeratin 5 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.Synonyms: 58 kDa Cytokeratin, CK5, Cytokeratin-5, K5, KRT5, Keratin 5, Keratin type II cytoskeletal 5, Keratin-5

Gene ID: 3852

NCBI Accession: NP_000415

UniProt: P13647