Dendritic Cells antibody
Reactivity: Human
IHC (fro)
Host: Mouse
Monoclonal
Ki-M4
unconjugated
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- Target
- Dendritic Cells
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Immunohistochemistry (Frozen Sections) (IHC (fro))
- Purification
- Protein A chromatography.
- Immunogen
- Circulating immune complexes of a patient with Hodgkin's disease.
- Clone
- Ki-M4
- Isotype
- IgG2a
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- Application Notes
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Immunohistochemistry on frozen sections: use at 0.5 μg/mL (1: 400).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Protocol
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum
- Restrictions
- For Research Use only
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- Reconstitution
- Restore with 0.5 mL distilled water.
- Concentration
- 0.2 mg/mL
- Buffer
- PBS buffer with 0.02 % sodium azide as preservative and 10 mg/mL BSA as stabilizer.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
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- Target
- Dendritic Cells
- Background
- Monoclonal antibody Ki-M4 detects the accessory B-cell macrophages in lymphoid organs.Synonyms: Dendritic cell marker
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