MHC Class II (I-A K,s,r) antibody
Reactivity: Mouse
FACS, IHC (fro)
Host: Rat
Monoclonal
ER-TR2
unconjugated
1 image
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- Target
- MHC Class II (I-A K,s,r)
- Reactivity
- Mouse
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Purification
- Affinity Chromatography
- Immunogen
- Murine thymic reticulum Remarks: MHC Class II antigens are heterodimers consisting of one alpha-chain (31-34kDa) and one beta-chain (26-29kDa). The epitope has not been further characterized.
- Clone
- ER-TR2
- Isotype
- IgG2b
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- Application Notes
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Immunohistochemistry on frozen sections (1 μg/mL, 1: 200). Suggested positive control: Mouse spleen. FACS. Does not react on routinely processed paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Protocol
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase
- Restrictions
- For Research Use only
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- Reconstitution
- Restore with 0.5 mL distilled water (=stock solution).
- Concentration
- 0.2 mg/mL
- Buffer
- Phosphate buffered saline, pH 7.2 (PBS), 0.01 % Thimerosal, 10 mg/mL BSA
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store at 2 - 8 °C.
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- Target
- MHC Class II (I-A K,s,r)
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