MHC Class II Antigen I Ak,d,b,q,r antibody (FITC)

Catalog No. ABIN111989

Product Details

Target: MHC Class II Antigen I Ak,d,b,q,r

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

No Cross-Reactivity: Human

Cross-Reactivity (Details): Species reactivity (tested):Mouse (Cells expressing MHC class II antigens.

Purification: Affinity Chromatography.

Immunogen: Murine thymic reticulum. Remarks: Antigen / Epitope: MHC Class II antigens are heterodimers consisting of one alpha-chain (31-34 kDa) and one beta-chain (26-29 kDa). The epitope has not been further characterized.

Clone: ER-TR3

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: 10 μg/mL (Use 10 μL of 1/10 diluted antibody to label 10^6 cells). Suggested Positive Control: mouse spleen. Has beed described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: Phosphate buffered saline pH 7.2 (PBS) containing 10 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as a preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details

Target: MHC Class II Antigen I Ak,d,b,q,r

Alternative Name: MHC Class II (I-A K,b,d,q,r)

Background: MHC Class II antigens are heterodimers consisting of one alpha chain (31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR 3, ER-TR 2, ER-TR 1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.