CD163 antibody (FITC)

Catalog No. ABIN111917

Product Details anti-CD163 Antibody (FITC)

Target: CD163

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD163 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: Monoclonal antibody 5C6 FAT recognizes a membrane Glycoprotein on Monocytes and Macrophages which is expressed in intermediate and late inflammatory stages. Antigen Distribution Isolated Cells: Monocytes, particularly after dexamethasone treatment or after 2-5 days in culture. Does not react with lymphocytes, granulocytes or platelets. Tissue Sections: Positive staining can be observed in the skin (histiocytes), gut, Kupffer cells, few alveolar macrophages, a major population of macrophages in the placenta, varying degrees of macrophages in inflamed tissues, including tumorous tissue depending on the inflammatory stage. Red pulp, but not white pulp macrophages of the spleen, and cortical macrophages of the thymus are detected. Macrophages in the synovialis of patients with rheumatoid arthritis. In alveolar macrophages and in Kupffer cells a double staining can be observed with monoclonal antibody 25F9 (product BM4022) which is not the case in other tissues. The antigen is also found abundantly in human term placenta, and regularly in acute and chronic inflammatory lesions.

Cross-Reactivity (Details): Species reactivity (tested):Human: Monocytes and Macrophages. Negative with Pig.

Purification: Affinity Chromatography

Immunogen: Human CD163. Remarks: The epitope has not been further characterized.

Clone: 5C6-FAT

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 50 μL of 1/10-1/20 diluted antibody to label 5x10e5 cells. Suggested Positive Control: Human monocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2, 0.05 % Sodium Azide, 10 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD163 antibody (FITC) (ABIN111917)

Bir, Calli-Demirkan, Tufan, Akbulut, Satiroglu-Tufan: "Apoptotic cell death and its relationship to gastric carcinogenesis." in: World journal of gastroenterology : WJG, Vol. 13, Issue 23, pp. 3183-8, (2007) (PubMed).

Zaba, Cardinale, Gilleaudeau, Sullivan-Whalen, Suárez-Fariñas, Suárez Fariñas, Fuentes-Duculan, Novitskaya, Khatcherian, Bluth, Lowes, Krueger: "Amelioration of epidermal hyperplasia by TNF inhibition is associated with reduced Th17 responses." in: The Journal of experimental medicine, Vol. 204, Issue 13, pp. 3183-94, (2007) (PubMed).

Target Details for CD163

Target: CD163

Alternative Name: CD163 (CD163 Products)

Synonyms: DMBT1 antibody, CD163 antibody, CD163v2 antibody, CD163v3 antibody, M130 antibody, MM130 antibody, deleted in malignant brain tumors 1 protein-like antibody, CD163 molecule antibody, CD163 antigen antibody, LOC426820 antibody, CD163 antibody, Cd163 antibody

Background: CD163 is a scavenger receptor for the haemoglobin-haptoglobin complex, and is upregulated by glucocorticoids and IL-10. The extracellular portion of the receptor is regularly shed and can be found in the circulation. An important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells. CD163-positive cells include skin histiocytes, Kupffer cells, spleen macrophages of the red pulp, and some thymus macrophages. The antigen is also found abundantly in human term placenta, and regularly in acute and chronic inflammatory lesions.Synonyms: Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine-rich type 1 protein M130

Gene ID: 9332

NCBI Accession: NP_004235

UniProt: Q86VB7