Macrophages antibody

Catalog No. ABIN111911

Product Details

Target: Macrophages

Reactivity: Human, Pig, Monkey, Dog, Cat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody Clone PM-2K, together with X-4 (BM4035) and X-14 (BM4036), forms a particular group of macrophage specific antibodies which were classified at the V Leukocyte Typing Workshop held in Boston in 1993. PM-2K recognises tissue macrophages and macrophages in proliferative disorders of macrophages. PM-2K is one of the markers that, being negative on dendritic cells, can differentiate between macrophages and dendritic cells. Under non-reducing conditions PM-2K recognizes an antigen of 150 kDa (Western blot) which has been shown to be on cytoplasmic membranes. Antigen Distribution: Isolated Cells: Positive on >90 % of alveolar macrophages, and on 10 % of adherent peritoneal cells after 1 day culture. Negative on dendritic cells, on freshly isolated blood monocytes or peritoneal cells, and on bone marrow cells including monocytes, myelomonocytic precursors and megakaryocytes. Tissue Sections: PM-2K stains most tissue macrophages in lymphoreticular organs such as thymus, spleen, lymph node and tonsil. It is positive on Kupffer cells of the liver, alveolar macrophages and macrophages in the interstitial tissues of the kidney, pancreas and many other organs. In Gaucher's disease multicentric reticulohistiocytosis and malignant histiocytosis proliferating macrophages are positive. In MFH infiltrating macrophages are stained but not tumour cells. Some bone stromal cells but not osteoclast-like multinucleated giant cells are stained in GCT. Microglial cells, osteoclasts and dendritic cells such as Langerhans cells, interdigitating cells and follicular dendritic cells are negative.

Cross-Reactivity (Details): Species reactivity (tested):Human (Macrophages).Positive on Macrophages of Cat, Dog, Pig, Bovine and Monkey.Negative in Rabbit, Rat, Guinea Pig, Gold Hamster, Goat, Horse.

Purification: Affinity Chromatography.

Immunogen: 24h cultivated Human peritoneal Macrophages

Clone: PM-2K

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on Frozen Sections: 0.5 μg/mL (1/400)Does not react on routinely processed Paraffin Sections. Suggested Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 with 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use

Storage: 4 °C

Storage Comment: Store the antibody at 2-8 °C as an undiluted liquid. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Target Details

Target: Macrophages

Background: Synonyms: Macrophage marker