Histone 3 antibody (C-Term)

Catalog No. ABIN1118467

Supplier Product No.: 100-401-e81

Product Details anti-Histone 3 Antibody

Target: Histone 3 (H3)

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Supplier Product No.: 100-401-e81

Supplier: Rockland

Purpose: Histone H3 Antibody

Cross-Reactivity (Details): Anti-Histone H3 is directed against the human histone 3 protein.

Characteristics: Synonyms: rabbit anti-Histone H3 antibody, Histone H3.1, H3F, H3FA, H3FB, H3FL, H3FL, H3FC, H3FD, H3FI, H3FH, H3FK, H3FF, H3FJ, Histone 3 Antibody

Purification: The product was prepared from monospecific antiserum by delipidation and defibrination.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Histone-3 was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to the c-terminus region of human histone-3.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:100

Application Note: Histone H3 antibody has been tested for use in ELISA, IHC, and western blot. For western blots expect a band of approximately 15.4 kDa in size corresponding to the Histone 3 protein. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:2000

ELISA Dilution: 1:10000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 83 mg/ml

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store H3 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Histone 3 antibody (ABIN1118467)

Rivera-Casas, Gonzalez-Romero, Garduño, Cheema, Ausio, Eirin-Lopez: "Molecular and Biochemical Methods Useful for the Epigenetic Characterization of Chromatin-Associated Proteins in Bivalve Molluscs." in: Frontiers in physiology, Vol. 8, pp. 490, (2017) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3)

Alternative Name: Histone H3 (H3 Products)

Background: Background: Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone proteins are highly post-translationally modified with Histone H3 being the most extensively modified of the five histones. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H3 Antibody is ideal for investigators involved in Cell Signaling, Epigenetics, Nuclear Signaling research and Signal Transduction research.

Gene ID: 8350

NCBI Accession: NP_003520

UniProt: P68431