Follicular Dendritic Cells antibody

Catalog No. ABIN111843

Product Details anti-Follicular Dendritic Cells Antibody

Target: Follicular Dendritic Cells

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Follicular Dendritic Cells antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity (Details): Species reactivity (tested):Rat.

Purification: Affinity Chromatography

Immunogen: Rat peritoneal macrophages.

Clone: Ki-M9R

Isotype: IgG1

Application Details

Application Notes: Suitable for Immunohistochemistry (IHC): Frozen sections: 5 μg/mL (1: 200)Paraffin sections: 50 μg/mL (1: 20), Proteinase K pretreatment for antigen retrieval isrecommended. Suggested positive control: Rat spleenAntigen Distribution Isolated cells: Ki-M9R is negative with all peripheral blood cells andcells of the bone marrow or peritoneal cavity.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG (H+L)minimal-cross reaction to rat) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalumProtocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Incubate the tissue section with proteinase K for 7min. 3. Wash in distilled water4. Block endogenous peroxidase5. Wash in PBS6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber8. Wash in PBS9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG (H+L)minimal-cross reaction to rat) for 1h in a humid chamber10. Wash in PBS11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 12. Wash in PBS13. Counterstain with Mayer's hemalum

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water (stock solution).

Concentration: 1.0 mg/mL

Buffer: PBS buffer with 0.01 % Thimerosal as preservative and 10 mg/mL BSA as stabilizer

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Target Details for Follicular Dendritic Cells

Target: Follicular Dendritic Cells

Background: Follicular dendritic cells are cells that facilitate antigen recognition by B cells in follicles. This kind of dendritic cell is not bone marrow derived and is not a kind of blood cell. It is purely a resident of the follicles of secondary lymphoid organs. B cells form germinal centers around follicular dendritic cells in lymphoid organs. Dendritic cells form from monocytes, white blood cells which circulate in the body and, depending on the right signal, can turn into dendritic cells or macrophages. The monocytes in turn are formed from stem cells in the bone marrow.