Macrophages antibody

Catalog No. ABIN111840

Product Details

Target: Macrophages

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects monocytes, subpopulation of macrophages, interdigitating cells. The antigen is localized on the surface of cells. It is stable to 2 % periodate-lysine-paraformaldehyde fixation. The epitope has not been further characterized. Antigen Distribution: Isolated cells: Positive on monocytes in peripheral blood. Positive on 20-30 % of peritoneal cells, increasing to 30-40 % after thioglycolate stimulation. Tissue sections: The antigen is found on a subpopulation of most tissue fixed macrophages. It is quite predominant on red pulp macrophages and interdigitating cells in the outer periarteriolar lymphatic sheath of the spleen, the inner border of marginal sinus of lymph nodes, and alveolar macrophages. Langerhans cells of the skin and twisted spindle-shaped cells of the omentum are detected by the antibody. It is absent from sinusoidal endothelial cells and parenchymal cells.

Cross-Reactivity (Details): Species reactivity (tested):Rat (Monocytes, subpopulation of Macrophages, interdigitating cells).

Purification: Affinity Chromatography.

Immunogen: Rat Peritoneal Macrophages.

Clone: RM1

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on Frozen Sections: 2 μg/mL (1/100). Immunohistochemistry on Paraffin Sections: 20 μg/mL (1/10). Microwave pretreatment forantigen retrieval is recommended. Has been described to work in FACS. Suggested Positive Control: Rat spleen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.2 mg/mL

Buffer: PBS pH 7.2 with 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2 - 8 °C.

Target Details

Target: Macrophages

Background: The antigen is found on a subpopullation of most tissue fixed macrophages. It is quite predominant on red pulp macrophages and interdigitating cells in the outer periarteriolar lymphatic sheath of the spleen, the inner border of marginal sinus of lymph nodes, and alveolar macrophages. Langerhans cells of the skin and twisted spindle-shaped cells of the omentum are detected by the antibody. It is absent from sinusoidal endothelial cells and parenchymal cells.Synonyms: Macrophage marker