HCV NS5 antibody (AA 2190-2300)

Catalog No. ABIN111768

Product Details anti-HCV NS5 Antibody

Target: HCV NS5 (Hepatitis C Virus NS5 (HCV NS5))

Binding Specificity: AA 2190-2300

Reactivity: Hepatitis C Virus (HCV)

Host: Mouse

Clonality: Monoclonal

Conjugate: This HCV NS5 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purification: Protein G Chromatography.

Immunogen: Recombinant antigen (HCV) genotype 1b. Remarks:

Clone: 7-D4

Isotype: IgG1

Application Details

Application Notes: Suitable for use in ELISA (approx. 10e-6), Western Blot (1/1000) and Immunohistochemistry(Cryostat liver sections: 1/20).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 0.01 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Do not freeze working dilutions

Target Details for HCV NS5

Target: HCV NS5 (Hepatitis C Virus NS5 (HCV NS5))

Alternative Name: HCV NS5 (HCV NS5 Products)

Target Type: Viral Protein

Background: HCV is classified into the genus Hepacivirus of the family Flaviviridae. Like all the members of the family, HCV is an enveloped, single-stranded, positive-sense RNA virus. Its genome (about 9600 nt) is flanked at both termini by conserved, highly structured non-translated regions (NTRs) and encodes a polyprotein precursor (about 3000 aa), which is proteolytically processed by host and viral proteases to produce the structural (core, E1, E2 and p7) and non-structural (NS2, NS3, NS4A, NS4B, NS5A and NS5B) proteins of the virus. Recently, an additional protein has been identified, whose function remains unknown. NS5A is a ~56 kDa pleiotropic protein with key roles in both viral RNA replication and modulation of the physiology of the host cellSynonyms: Hepatitis C Virus Non-structural protein NS5A