HCV E1 antibody

Catalog No. ABIN111760

Product Details anti-HCV E1 Antibody

Target: HCV E1 (Hepatitis C Virus Envelope Protein E1 (HCV E1))

Reactivity: Hepatitis C Virus (HCV)

Host: Mouse

Clonality: Monoclonal

Conjugate: This HCV E1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purification: Protein A Chromatography (Source: Ascites).

Immunogen: Recombinant HCV E1 glycoprotein (genotype 1b).

Clone: BDI198

Isotype: IgG

Application Details

Application Notes: IFA. ELISA. Western blot. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 2.0 mg/mL (OD280 nm, E0.1% = 1.3)

Buffer: 0.01 M PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative without stabilizing proteins.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for HCV E1

Target: HCV E1 (Hepatitis C Virus Envelope Protein E1 (HCV E1))

Alternative Name: HCV Envelope Glycoprotein E1 (HCV E1 Products)

Target Type: Viral Protein

Background: Envelope glycoproteins E1 and E2 are involved in virus attachment to the host cell as well as in virus endocytosis and fusion with host membrane. E2 inhibits human EIF2AK2/PKR activation, preventing the establishment of an antiviral state. E2 is a viral ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on liver sinusoidal endothelial cells and macrophage-like cells of lymph node sinuses. These interactions allow capture of circulating HCV particles by these cells and subsequent transmission to permissive cells. DCs are professional antigen presenting cells, critical for host immunity by inducing specific immune responses against a broad variety of pathogens. They act as sentinels in various tissues where they entrap pathogens and convey them to local lymphoid tissue or lymph node for establishment of immunity. Capture of circulating HCV particles by these SIGN+ cells may facilitate virus infection of proximal hepatocytes and lymphocyte subpopulations and may be essential for the establishment of persistent infection.Synonyms: Heptitis C Virus E1, gp32, gp35