Clathrin Heavy Chain (CLTC) (Heavy Chain) antibody
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- Target See all Clathrin Heavy Chain (CLTC) Antibodies
- Clathrin Heavy Chain (CLTC)
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Binding Specificity
- Heavy Chain
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Reactivity
- Human, Rat, Cow, Pig, Amphibian
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- Clone CHC5.9 represents an excellent marker for detection of receptor mediated endocytosis (for review cf. e.g. Refs. 2 and 3). Polypeptide Reacting: Mr 180 000 polypeptide (clathrin heavy chain) from coated vesicles of various tissues. Structures and Tissues Specifically Detected: Coated vesicles of different organs and tissues (eg brain, mammary gland, ovaries). Reactivities on Cultured Cell Lines (tested so far): HeLa, SV-40, RVF-SMC.
- Cross-Reactivity (Details)
- Species reactivity (tested):Human, Bovine, Pig, Rat and Amphibia.
- Purification
- Gel filtration
- Immunogen
- Coated vesicles (Clathrin) of Bovine brain.
- Clone
- CHC5-9
- Isotype
- IgM
- Top Product
- Discover our top product CLTC Primary Antibody
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anti-Clathrin Heavy Chain (CLTC) antibody
CLTC Reactivity: Human WB, IHC, IF, IP Host: Rabbit Monoclonal unconjugated
anti-Clathrin Heavy Chain (CLTC) antibodyCLTC Reactivity: Human, Mouse, Rat, Cow, Pig WB, ELISA, FACS, IP, ICC Host: Mouse Monoclonal BF-06 unconjugated
anti-Clathrin Heavy Chain (CLTC) antibodyCLTC Reactivity: Human, Mouse, Rat WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Clathrin Heavy Chain (CLTC) (N-Term) antibodyCLTC Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Monkey, Avian, Chicken, Horse, Rabbit, Cat, Donkey, Goat, Guinea Pig, Hamster, Sheep WB, IF Host: Goat Polyclonal unconjugated
anti-Clathrin Heavy Chain (CLTC) (AA 1019-1048) antibodyCLTC Reactivity: Human WB, FACS, IHC (p) Host: Rabbit Polyclonal RB24434 unconjugated
anti-Clathrin Heavy Chain (CLTC) (AA 1617-1630) antibodyCLTC Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Monkey, Xenopus laevis, Chicken, Horse, Rabbit, Bat, Zebrafish (Danio rerio) IHC, IHC (p) Host: Goat Polyclonal unconjugated
anti-Clathrin Heavy Chain (CLTC) (AA 232-340) antibodyCLTC Reactivity: Human WB, ELISA, PLA Host: Mouse Monoclonal 2E5 unconjugated
anti-Clathrin Heavy Chain (CLTC) (AA 851-990) antibodyCLTC Reactivity: Human ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Clathrin Heavy Chain (CLTC) (AA 401-500) antibodyCLTC Reactivity: Human, Mouse WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Clathrin Heavy Chain (CLTC) (AA 232-340) antibodyCLTC Reactivity: Human WB, ELISA Host: Mouse Polyclonal unconjugated
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- Application Notes
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Immunohistochemistry on Frozen Tissue: 1/10. (When reconstituted, dilute further with PBS, pH 7.4). Incubation Time: 1 h at RT. Immunoblotting (Western): 1/100-1/500 (ECL). Successful use in Immunoprecipitation was reported by some researchers. As thisdetection method has not been verified by antibodies-online, the application was deletedfrom the database. This does not necessarily exclude the use in such procedure.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore with 1 mL distilled water.
- Storage
- 4 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C.
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- Target
- Clathrin Heavy Chain (CLTC)
- Alternative Name
- Clathrin Heavy Chain (CLTC Products)
- Background
- Clathrin is a protein which assembles into a polyhedral network on the cell membrane as the membrane invaginates, forming a coated pit which is essential to endocytosis. Clathrin is composed of three polypeptides, a 180 kDa heavy chain and two 32-38 kDa light chains which combine to create a distinct three-legged triskelion. It is this morphology which allows Clathrin to form its unique polyhedral network.Synonyms: CLH17, CLTC, CLTCL2, Clathrin heavy chain on chromosome 17, KIAA0034, Membrane Vesicle Marker
- Gene ID
- 281080
- NCBI Accession
- NP_776448
- UniProt
- P49951
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