MBP Tag antibody

Catalog No. ABIN1112992

Product Details

Target: MBP Tag

Reactivity: Please inquire

Host: Mouse

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Immunogen: Recombinant MBP protein with full length.

Isotype: IgG1

Application Details

Application Notes: WB (1:1000-10,000). Other applications have not been tested. The optimal dilutions should be determined by end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Storage: 4 °C

Storage Comment: Store at 2-8 °C for 1 month. Or aliquot and store at -20 °C or below for long term. Avoid repeated freeze and thaw cycles.

Target Details

Target: MBP Tag

Target Type: Tag

Background: Maltose-Binding Protein (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It has an approximate molecular mass of 42.5 kilodaltons. MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins. The fusion protein binds to amylose columns while all other proteins flow through. The MBP-protein fusion can be purified by eluting the column with maltose.