TCR beta antibody (APC)

Catalog No. ABIN1112514

Product Details anti-TCR beta Antibody (APC)

Target: TCR beta (T Cell Receptor (TCR) beta (TCR beta))

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This TCR beta antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Immunogen: Affinity purified TCR from mouse DO-11.10 cells Species

Clone: H57-597

Isotype: IgG

Application Details

Application Notes: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of mouse tissue for flow cytometric analysis using 0,25 µg/10^6 cells

Comment:

Allophycocyanin (APC). Abs/Em: 651/662 nm.

Reagent Preparation:

1. Transfer the sample to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add anti-mouse anti-mouse TCR beta APC and mix gently with a vortex mixer. The optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive APC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 mL of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 mL 0.01 mol/L PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 mL PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

Preservative: Sodium azide

Precaution of Use: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Storage: 4 °C

Target Details for TCR beta

Target: TCR beta (T Cell Receptor (TCR) beta (TCR beta))

Alternative Name: TCR beta (TCR beta Products)

Synonyms: TCRbeta antibody, Tib antibody, T cell receptor beta chain antibody, Tcrb antibody

Background: T cell receptor (TCR) is a heterodimer consisting of an alpha and a beta chain (TCR alpha/beta) or a gamma and a delta chain (TCR gamma/delta). TCR-beta is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-alpha). It is expressed on alpha/beta TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.