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CD45.2 antibody (APC)

Reactivity: Mouse FACS, IF Host: Mouse Monoclonal Ks20-8 APC
Catalog No. ABIN1112456
  • Target See all CD45.2 products
    CD45.2 (CD45 (CD45.2 Allogen))
    Reactivity
    Mouse
    Host
    • 53
    Mouse
    Clonality
    • 53
    Monoclonal
    Conjugate
    • 7
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD45.2 antibody is conjugated to APC
    Application
    • 53
    • 13
    • 10
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF)
    Characteristics
    Mouse monoclonal Anti-Mouse CD45.2 APC is recommended for use in flow cytometry for identification of mouse CD45.2, an alloantigen of CD45 expressed by Ly5.2 bearing mouse strains.
    Immunogen
    B10.S mouse thymocytes and splenocytes
    Clone
    Ks20-8
    Isotype
    IgG2a
  • Application Notes
    It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of mouse tissue for flow cytometric analysis using less than or equal to less than or equal to 0,25 µg/10^6 cells in 100 µL volumeIt is recommended that the reagent be titrated for optimal performance for each application.
    Comment

    Allophycocyanin (APC). Abs/Em: 651/ 662 nm.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The conjugate is provided in liquid form in buffer containing PBS and 0,09% Sodium azide, pH 7.2.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
  • Target
    CD45.2 (CD45 (CD45.2 Allogen))
    Alternative Name
    CD45.2 (CD45.2 Products)
    Background
    CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kDa glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell, as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
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