CD45 antibody (APC)
-
- Target See all CD45 (PTPRC) Antibodies
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This CD45 antibody is conjugated to APC
-
Application
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)
- Characteristics
- Monoclonal Mouse Anti-Human CD45 is recommended for use in flow cytometry for identification and analysis of CD45 cells.
- Clone
- HI30
- Isotype
- IgG1
- Top Product
- Discover our top product PTPRC Primary Antibody
-
-
- Application Notes
- It is recommended for use in flow cytometry and imunohistochemistry reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.
- Comment
-
Allophycocyanin (APC), Abs/Em: 651/662 nm.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- The conjugate is provided in liquid form in buffer containing and Antibody Stabilizer solution, PBS 20 mM, pH 7,4.
- Preservative
- Sodium azide
- Precaution of Use
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
- Storage
- 4 °C
-
-
Oncogene MYCN regulates localization of NKT cells to the site of disease in neuroblastoma." in: The Journal of clinical investigation, Vol. 117, Issue 9, pp. 2702-12, (2007) (PubMed).
: "Mobilization of bone marrow-derived stem cells after myocardial infarction and left ventricular function." in: European heart journal, Vol. 26, Issue 12, pp. 1196-204, (2005) (PubMed).
: "
-
Oncogene MYCN regulates localization of NKT cells to the site of disease in neuroblastoma." in: The Journal of clinical investigation, Vol. 117, Issue 9, pp. 2702-12, (2007) (PubMed).
-
- Target
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
- Alternative Name
- CD45 (PTPRC Products)
- Synonyms
- PTPRC antibody, si:dkeyp-47c5.1 antibody, B220 antibody, CD45 antibody, CD45R antibody, GP180 antibody, L-CA antibody, LCA antibody, LY5 antibody, T200 antibody, Cd45 antibody, Ly-5 antibody, Lyt-4 antibody, loc antibody, Lca antibody, RT7 antibody, cd45 antibody, protein tyrosine phosphatase, receptor type C antibody, protein tyrosine phosphatase, receptor type, C antibody, receptor-type tyrosine-protein phosphatase C antibody, PTPRC antibody, ptprc antibody, LOC100074271 antibody, Ptprc antibody
- Background
- CD45 is a 180-240 kDa single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA), B220, and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.
- Pathways
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling
-