PDGFRB antibody

Catalog No. ABIN111188

Product Details anti-PDGFRB Antibody

Target: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This PDGFRB antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Specificity: This antibody is specific for the extracellular domain of the PDGF receptor beta subunit. This was confirmed in binding studies using several different cell lines and by its ability to immunoprecipitate PDGF receptor beta complexed with 125I-PDGF-BB. This antibody does not recognize PDGF receptor alpha. Binding of PDGF-BB induces receptor homodimerization or heterodimerization with PDGF receptor alpha.

Purification: Affinity chromatography on Protein G

Immunogen: Human skin fibroblast cell membrane extracts.

Clone: 21-72

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 25 μg/mL. Immunoprecipitation. Western Blot. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 25 μg/mL. Recommended Positive Control: Human breast cancer tissue.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PDGFRB

Target: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Alternative Name: CD140b / PDGFRB (PDGFRB Products)

Background: PDGF receptor beta is a member of the class III receptor tyrosine kinase family that also includes M-CSF receptor, SCF receptor and Flt-3.Synonyms: Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, PDGF Receptor beta, PDGF-R-beta

Gene ID: 5159

NCBI Accession: NP_002600

UniProt: P09619

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling