Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detected: all adenocarcinoma, mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide reacting: Mr 45 000 polypeptide (human keratin K18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia. Reactivities on cultured cell lines (tested so far): HeLa and MCF-7
Cross-Reactivity (Details)
Species reactivity (tested):Human, Mouse, Rat, Bovine, Porcine, Canine, Hamster, Sheep, Fish (Trout) and Zebrafish
Purification
Protein A chromatography
Immunogen
Human keratin K18 from HeLa cytoskeletal preparation
Western blot. Immunohistochemistry (dilute 1: 20 with PBS): suitable for cytological material, frozen andparaffin-embedded tissue (for best performance, microwave treatment is recommended). Incubation time: 1h at 37C, extended with paraffin sections (overnight at 2-8C). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in 1.0 mL distilled water.
Buffer
PBS, pH 7.4, 0.09 % Sodium azide, 0.5 % BSA
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing). Dilute only prior to immediate use.
Storage
-20 °C
Storage Comment
Store the antiserum at -20 °C (aliquot contents to
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CK18 antibody, K18 antibody, Krt1-18 antibody, CYK18 antibody, DreK18 antibody, cb83 antibody, dapk1 antibody, sb:cb83 antibody, wu:fa13f02 antibody, wu:fb36b04 antibody, krt18 antibody, MGC64569 antibody, MGC75922 antibody, KRT18 antibody, CK-18-A antibody, K18-A antibody, NC-11 antibody, cyk18 antibody, k18 antibody, krt18-a antibody, krt18-b antibody, krt18a antibody, xkendob antibody, keratin 18 antibody, keratin 18, type I L homeolog antibody, keratin 18, type I antibody, keratin, type I cytoskeletal 18 antibody, K18, simple type I keratin antibody, Keratin, type I cytoskeletal 18 antibody, keratin 18, type I S homeolog antibody, Krt18 antibody, KRT18 antibody, krt18 antibody, krt18.L antibody, LOC700569 antibody, LOC100136778 antibody, k1c18 antibody, LOC100008885 antibody, krt18.S antibody
Background
Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18