P-Selectin antibody (APC)

Catalog No. ABIN1111105

Product Details anti-P-Selectin Antibody (APC)

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This P-Selectin antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Specificity: The antibody AK4 recognizes an extracellular epitope of CD62P (P-selectin), a 140 kD single chain type I transmembrane glycoprotein present in secretory alpha-granules in platelets, in Weibel-Palade bodies in endothelial cells and in megakaryocytes, it is relocated to the plasma membrane upon activation.

Cross-Reactivity (Details): Human, Non-Human Primates

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Human platelets

Clone: AK4

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-P-Selectin antibody (APC) (ABIN1111105)

Ludwig, Schultz, Boehncke, Podda, Tandi, Krombach, Baatz, Kaufmann, von Andrian, Zollner: "Activated, not resting, platelets increase leukocyte rolling in murine skin utilizing a distinct set of adhesion molecules." in: The Journal of investigative dermatology, Vol. 122, Issue 3, pp. 830-6, (2004) (PubMed).

Kowalska, Ratajczak, Hoxie, Brass, Gewirtz, Poncz, Ratajczak: "Megakaryocyte precursors, megakaryocytes and platelets express the HIV co-receptor CXCR4 on their surface: determination of response to stromal-derived factor-1 by megakaryocytes and platelets." in: British journal of haematology, Vol. 104, Issue 2, pp. 220-9, (1999) (PubMed).

Dunlop, Skinner, Bendall, Favaloro, Castaldi, Gorman, Gamble, Vadas, Berndt: "Characterization of GMP-140 (P-selectin) as a circulating plasma protein." in: The Journal of experimental medicine, Vol. 175, Issue 4, pp. 1147-50, (1992) (PubMed).

Target Details for P-Selectin

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Alternative Name: CD62P (SELP Products)

Synonyms: PSELECT antibody, CD62 antibody, CD62P antibody, GMP140 antibody, GRMP antibody, LECAM3 antibody, PADGEM antibody, PSEL antibody, GMP-140 antibody, Grmp antibody, selectin P antibody, selectin, platelet antibody, Selp antibody, SELP antibody

Background: Selectin P,CD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.,P-selectin, GMP140, SELP, PADGEM, GRMP, LECAM3, PSEL

Gene ID: 6403

UniProt: P16109