CHMP3 antibody

Catalog No. ABIN1109497

Product Details anti-CHMP3 Antibody

Target: CHMP3 (Charged Multivesicular Body Protein 3 (CHMP3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHMP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificity: Reacts with 25 kDa hVps24.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein A

Immunogen: Synthetic peptide derived from internal domain of Human Vps24 protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in distilled water.

Buffer: 0.1 M Tris, 0.1 M Glycine and 2 % Sucrose, None

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store the antibody at -20 °C. Store reconstituted antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CHMP3

Target: CHMP3 (Charged Multivesicular Body Protein 3 (CHMP3))

Alternative Name: VPS24 (CHMP3 Products)

Background: The charged multivesicular body proteins, commonly designated CHMPs, belong to the vacuolar sorting protein family and function as chromatin-modifying proteins. CHMP1-6 are all components of ESCRT (endosomal sorting complex required for transport) I, II or III complexes. These complexes are crucial for sorting endosomal articles into multivesicular bodies (MVBs), as well as required for the formation of these bodies. CHMP3, also known as Vps24, associates directly with CHMP2 and CHMP4 for the disassembly of ESCRT-III complex in an ATP-dependent manner. During HIV-1 infection, the virus uses the ESCRT-III complex to mediate budding and exocytosis of viral proteins. Overexpression of CHMP3 strongly inhibits HIV-1 release. CHMP3 is expressed in brain, skeletal muscle, heart, lung, kidney and liver.Synonyms: CGI-149, CHMP3, Charged multivesicular body protein 3, Chromatin-modifying protein 3, NEDF, Neuroendocrine differentiation factor, Vacuolar protein-sorting-associated protein 24

Gene ID: 51652

NCBI Accession: NP_001005753