Ubiquitin antibody

Catalog No. ABIN1109442

Product Details anti-Ubiquitin Antibody

Target: Ubiquitin

Reactivity: Human, Mouse, Rat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody reacts with erythrocyte ubiquitin. Clone 2C5 does not react with multi ubiquitin.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat, bovine

Purification: Ammonium sulfate precipitation and protein A agarose

Immunogen: Bovine erythrocyte ubiquitin

Clone: 2C5

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Prepare a stock solution by dissolving the lyophilized antibody in 100 μL of distilled water. After reconstitution, the IgG concentration will be 1 mg/mL.

Concentration: 1.0 mg/mL

Buffer: PBS ( pH 7.2)/ 1 % sucrose

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store (in aliquots) at -20 °C.

Target Details for Ubiquitin

Target: Ubiquitin

Alternative Name: Ubiquitin (Ubiquitin Products)

Background: Ubiquitin is a polypeptide of 76 amino acid residues, and widely distributed protein in eukaryotic cells. This protein is also highly conserved among eukaryotic cells. There are several reports showed that intracellular abnormal and short-lived proteins are degraded through an ubiquitin dependent proteolytic pathway. In the ubiquitin dependent pathway, a target protein is tagged with multi-ubiquitin molecules.Synonyms: Polyubiquitin-B, UBB

Gene ID: 7314

UniProt: P0CG47

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway